The Passive Lysis Solution (PLS) is a mammalian cell lysis solution intended to be used to prepare cell lysates for measurement of luciferase activity with the twinlite and sensilite reporter gene assay systems. It is provided in the twinlite and Sensilite kits, but if additional volume of the lysis solution is required, it is also available as a separate item, 100 mL volume per bottle.
sensilite is an Enhanced Flash Firefly luciferase detection assay system designed to detect and quantitate Firefly luciferase from cultured cells with optimum sensitivity. The assay requires the use of a luminometer equipped with a dispenser as the signal has to be read shortly after dispensing.
twinlite is a dual luciferase reporter gene assay system designed to detect and quantitate both Firefly and Renilla luciferase sequentially from cultured cells. It requires the use of a luminometer equipped with two dispensers as the signal has to be read shortly after dispensing.